CORE SERVICES –NUCLEAR SIGNALLING, DNA DAMAGE, CYTOTOXICITY & PROLIFERATION ASSAYS

Nuclear signaling and nuclear material are immensely significant as they regulate and control cellular processes. Impediments in the nuclear signaling and/or DNA damage could grievously affect the cellular processes and hence the whole system.
DNA damage causes genetic instability which may increase the rate of cancer development, as recent findings make it increasingly clear that deficiencies in DNA-damage signaling and repair pathways are fundamental to the etiology of most, if not all, human cancers.
Cell proliferation is a vital concept with their rates being good indicators of cell health. Proliferation/viability analysis is also critical for cell growth and differentiation studies. Furthermore, assessing compound cytotoxicity is crucial in the development of pharmaceuticals. In cancer biology, these assays are used during the drug development process to evaluate both compound cytotoxicity and tumor inhibition.
Bearing these significant implications in mind, DR YELLAPRAGADA LIFESCIENCES provides a range of services pertinent to these concerns.

CAPABILITIES –NUCLEAR SIGNALLING, DNA DAMAGE, CYTOTOXICITY & PROLIFERATION ASSAYS

CORE SERVICES - APOPTOSIS, PYROPTOSIS & NECROSIS ASSAYS

The last decade witnessed an enigmatic discovery that apoptosis and the genes that control it greatly affect the malignant phenotype. While some oncogenic mutations disrupt apoptosis, leading to tumor initiation, progression, or metastasis, others promote apoptosis, generating selective pressure to override apoptosis during multistage carcinogenesis. Intense research efforts are unraveling the underlying mechanisms of apoptosis, envisioning the use of this information to produce novel strategies for exploiting apoptosis for therapeutic effects.
On the other hand, necrotic cell death in a tumor is suggestive of fast-growing cancer and is also utilized as a prognostic marker in several carcinomas. Given the significance of tumor necrosis and apoptosis in cancer progression and treatment, it is imperative to understand the underlying mechanisms of cell death and to distinguish between the two for cancer therapy development.
Pyroptosis is characteristically inflammatory and is triggered by various pathological stimuli viz., stroke, heart attack, or cancer. Besides, it is vital in controlling microbial infections.
Understanding the relevance of these aspects in modern cell biology and cancer biology research, DR YELLAPRAGADA LIFESCIENCES delivers far-reaching validated assays for apoptosis, pyroptosis, and necrosis.

CAPABILITIES - APOPTOSIS, PYROPTOSIS & NECROSIS ASSAYS

CORE SERVICES – CANCER SIGNALLING PATHWAYS & PHENOTYPIC ASSAYS

Cancer research is at the forefront of biological research specialties. Cancer signaling pathways and phenotypic assays form the core of cancer biology research. The current research scenario is witnessing a resurgence of interest in phenotypic screens in drug discovery as an alternative to target-focused approaches. With oncology being one of the most active therapeutic specialties, phenotypic assays pertinent to this area are of tremendous worth. With this advanced research interest in mind, DR YELLAPRAGADA LIFESCIENCES provides specialized and validated cancer signaling pathways and phenotypic assays.

CAPABILITIES-CANCER SIGNALLING PATHWAYS & PHENOTYPIC ASSAYS

CORE SERVICES – TOXICITY ASSAYS

Toxicity studies are vital in preclinical drug testing, and cell culture could be utilized for in vitro toxicity screening, either by estimation of the basal cell functions or by tests on specialized cell functions. Toxicity tests are mainly aimed at the detection of biological activity of the test substances on different cell types, where several parameters form the end-points of toxicity measurement. Organ-specific toxic effects are tested using specialized cells.

CAPABILITIES – TOXICITY ASSAYS

CORE SERVICES – INFLAMMATION, ANGIOGENESIS & METASTASIS ASSAYS

Among the several important factors pertinent to cell-based and cancer biology research, inflammation, angiogenesis, and metastasis are a significant few. Inflammation is a crucial feature in cancer, as the mediators and cellular effectors of inflammation are vital components of the local environment of tumors. While in some types of cancer, inflammation is observed before malignancy, the converse condition is observed in certain other types. Either way, inflammation in the tumor microenvironment has a plausible role in the promotion of tumor development, angiogenesis, and metastasis. This understanding has enhanced the identification of new targets that could lead to improved diagnosis and treatment. Also, cell-based research pertinent to stem cells and regenerative medicine finds these aspects to be extremely vital. Realizing this enigmatic importance of inflammation, angiogenesis & metastasis, DR YELLAPRAGADA LIFESCIENCES has surged ahead with its comprehensive research services in this area.

CAPABILITIES-INFLAMMATION, ANGIOGENESIS & METASTASIS ASSAYS

CORE SERVICES – FUNCTIONAL CELL STRESS ASSAYS

The metabolic signals promote cell growth as cells respond to these signals. Furthermore, cells also respond to exogenous stress through various adaptive stress response pathways. The activation and inactivation of these cell stress pathways can be studied through cell-based assays that utilize rapid and consistent reporters. With the intent of reliable drug efficacy studies on cell stress, DR YELLAPRAGADA LIFESCIENCES provides a range of validated cell-based assays, all of which can be specifically adapted to your desired cellular model.

CAPABILITIES- FUNCTIONAL CELL STRESS ASSAYS

STEM CELL-BASED ASSAYS

Isolation and maintenance of Adult Stem cells and umbilical cord blood/tissue stem cells
Characterization of MSCs/AMSCs/HSCs/NSCs
Neuronal differentiation studies
Osteogenic differentiation studies
Adipogenic differentiation studies
Dendritic and other immune cell generation from HSCs/MSCs
Signaling pathways governing stem cells: Notch, Wnt, Hedgehog, etc.

FLOW CYTOMETRY-BASED STUDIES

ADME

In Vitro ADME (Absorption, Distribution, Metabolism, Excretion)
In vitro, ADME studies help in the optimization of pharmacokinetic characteristics of a drug candidate identify the metabolic pathways and provide valuable inputs for the design of in vivo studies. We offer both standardized as well as customized assays.

Permeability (Caco2, MDCK, and PAMPA)
Aqueous Solubility
Metabolic stability and biotransformation
Test systems (microsomes, hepatocytes, S9, recombinant enzymes)
Species (rodent, guinea pig, rabbit, canine, non-human primate, human)
Protein binding (Equilibrium Dialysis and Ultrafiltration)
CYP Induction
CYP Isozyme Mapping
CYP Inhibition
Metabolite ID and profiling
Mass balance and drug disposition