CELL BASED ASSAYS
Cell-based assays are a vital early step in the testing of your compounds and drug candidates for activity.
- Proprietary Cell-Based Assays
- High-throughput Fluorescence based assays
- Primary Cells and Proprietary Cell based assays
CELL ASSAYS
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ANGIOGENESIS
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APOPTOSIS
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CELL MIGRATION
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CELL CYCLE ANALYSIS (FLOW CYTOMETER)
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CELL VIABILITY (MTT, SRB, CYQUANT, 3H)
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PROTEIN BINDING (EQUILIBRIUM DIALYSIS AND ULTRAFILTRATION)
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INFLAMMATION AND CYTOKINES ASSAYS
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REPORTER GENE ASSAYS (LUCIFERASE)
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GLUCOSE UPTAKE
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CELL & DIAGNOSTIC APPLICATIONS
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Cell cycle analysis
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Cell proliferation assays
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Apoptosis assays
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Mitochondrial membrane potential analysis
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Oxidative burst assay
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Reactive oxygen species analysis
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Immunofluorescence (confocal microscopy)
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Cell isolation and expansion
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Cell immortalization
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Cell sorting services
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Hematopoietic stem cell analysis
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CORE SERVICES – ENERGY METABOLISM & AUTOPHAGY ASSAYS
Energy metabolism, the central biochemical activity in all biological systems, comprises the overall network of enzyme-catalyzed pathways and involves the maintenance of the cellular and systemic viability of the organism. However, deviation from the normal, in the number of metabolites produced and/or the activity of the enzymes involved, causes several biochemical disorders. For this reason, energy metabolism assays are of utmost importance. Autophagy is a vital cellular response to stress or starvation. In the recent past, autophagy assays have witnessed unprecedented prominence in research and diagnosis of several diseases including cancer, due to their suggested physiological and pathophysiological roles. Considering the tremendous significance of energy metabolism and autophagy assays in cancer metabolism and signal transduction, DR YELLAPRAGADA LIFESCIENCES provides outstanding research services in this regard.
CAPABILITIES – ENERGY METABOLISM & AUTOPHAGY ASSAYS
| NAD(P)H assay |
| Lysosome detection assay |
| FAD assay |
| mTOR activity assay |
| ATP content assay |
| Poly-ubiquitination assay |
| Proteasome activity assay |
| Reactive Oxygen Species (ROS) test |
| cAMP assay |
| LC3B autophagy assay |
| Lipid peroxidation |
| LDL uptake test |
| Intracellular Glutathione (GSH) content |
| Lipids and phospholipids accumulation |
| P-glycoprotein (P-gp) activity assay |
| Anti-diabetic assays: Glucose uptake assay, α-amylase inhibition assay |
| Anti-diabetic - α-glucosidase inhibition assay, sucrose inhibition assay |
CORE SERVICES – FLOW CYTOMETRY FOR BIOMARKER DETECTION
Biomarker detection is central to any modern diagnostic methodology. A biomarker is a measurable indicator of the presence and/or severity of a particular disease state in an organism. Biomarkers have, thus, tremendously impacted diagnostic and prognostic methodologies in recent times. Apart from this, the property of biomarkers is also exploited in advanced cell-based research, including the detection of stem cell markers which find enormous applications and implications in stem cell research and regenerative medicine.
CAPABILITIES - FLOW CYTOMETRY FOR BIOMARKER DETECTION
| Surface and intracellular markers analysis in umbilical cord blood samples and PBMCs |
| Stem cell markers analysis viz., CD13, CD29, CD34, CD44, CD73, CD93, CD105, CD146 etc |
| Analysis of cell characterization using cell surface markers. |
| Analysis of GPCRs expression levels for antitumor drug discovery |
| Diagnosis of hematological disorders, cancer, infectious diseases, and genetic disorders |
| Detection and quantification of circulating endothelial cells & endothelial progenitor cells |
CORE SERVICES – CARDIAC BIOMARKERS DETECTION FOR MYOCARDIAL INFARCTION
Cardiovascular diseases (CVDs) are one of the leading causes of death worldwide. Recent times have witnessed an enormous increase in the rate of myocardial infarction owing to various factors. Cardiac biomarkers play a key role in the detection and prognosis of myocardial infarction. Intending to provide reliable and outstanding diagnostic and research services, DR YELLAPRAGADA LIFESCIENCES runs rapid and accurate cardiac biomarker detection services for myocardial infarction.
CAPABILITIES -CARDIAC BIOMARKERS DETECTION FOR MYOCARDIAL INFARCTION
| B-type Natriuretic peptide (BNP) assay |
| Creatine Kinase-MB (CK-MB) assay |
| Troponin assay |
| Heart-type fatty acid–binding protein (H-FABP) assay |
| Diagnosis of hematological disorders, cancer, infectious diseases, and genetic disorders |
| Myoglobin assay |
